mcl1 untagged Search Results


mcl  (OriGene)
89
OriGene mcl
Mcl, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcl/product/OriGene
Average 89 stars, based on 1 article reviews
mcl - by Bioz Stars, 2026-03
89/100 stars
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vector expressing mcl 1  (OriGene)
90
OriGene vector expressing mcl 1
bok/bax double deficiency causes greater sensitivity to NMDA- and OGD-induced neuronal death. A, bax−/−/bok+/+ and bax−/−/bok−/− cortical neurons were used to confirm Bok and Bax protein absence in culture by Western blotting. β-Actin was used as a loading control. B, Western blot and densitometry analysis comparing the levels of several anti-apoptotic members of the Bcl-2 family proteins, including Bcl-2, Bcl-w, Bcl-xL, and <t>Mcl-1</t> in bax−/−/bok+/+ and bax−/−/bok−/− cortical neurons. β-Actin was used as a loading control. Experiments were repeated three times with different preparations and with similar results. Means ± SEMs are shown. *p ≤ 0.05. p = 0.021 (Mcl-1) versus bax−/−/bok+/+ cultures (ANOVA, post hoc Tukey's test). C, D, Cortical neurons from bax−/−/bok+/+ and bax−/−/bok−/− mice were either treated with STS (100 nm) or exposed to the following: 100 μm NMDA for 5 min; 300 μm NMDA for 60 min; OGD for 90 min; or sham conditions for 90 min. Controls were the pool of DMSO and sham conditions for 24 h and 5 min, respectively. After treatments, cortical neurons were allowed to recover for 24 h. Cell death was assessed by Hoechst 33258 and PI staining, and PI-positive nuclei were scored as dead neurons and expressed as a percentage of the total population. Three subfields containing 300–400 neurons each were captured, and n = 9 wells were analyzed per condition from three separate cultures. Means ± SEMs are shown. *p ≤ 0.05. p values are the following: p = 0.0011 (STS), p = 0.0092 (NMDA at 100 μm, 5 min), p = 0.0053 (NMDA at 300 μm, 60 min), p = 0.018 (OGD) versus bax−/−/bok+/+ cultures (ANOVA, post hoc Tukey's test).
Vector Expressing Mcl 1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vector expressing mcl 1/product/OriGene
Average 90 stars, based on 1 article reviews
vector expressing mcl 1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


bok/bax double deficiency causes greater sensitivity to NMDA- and OGD-induced neuronal death. A, bax−/−/bok+/+ and bax−/−/bok−/− cortical neurons were used to confirm Bok and Bax protein absence in culture by Western blotting. β-Actin was used as a loading control. B, Western blot and densitometry analysis comparing the levels of several anti-apoptotic members of the Bcl-2 family proteins, including Bcl-2, Bcl-w, Bcl-xL, and Mcl-1 in bax−/−/bok+/+ and bax−/−/bok−/− cortical neurons. β-Actin was used as a loading control. Experiments were repeated three times with different preparations and with similar results. Means ± SEMs are shown. *p ≤ 0.05. p = 0.021 (Mcl-1) versus bax−/−/bok+/+ cultures (ANOVA, post hoc Tukey's test). C, D, Cortical neurons from bax−/−/bok+/+ and bax−/−/bok−/− mice were either treated with STS (100 nm) or exposed to the following: 100 μm NMDA for 5 min; 300 μm NMDA for 60 min; OGD for 90 min; or sham conditions for 90 min. Controls were the pool of DMSO and sham conditions for 24 h and 5 min, respectively. After treatments, cortical neurons were allowed to recover for 24 h. Cell death was assessed by Hoechst 33258 and PI staining, and PI-positive nuclei were scored as dead neurons and expressed as a percentage of the total population. Three subfields containing 300–400 neurons each were captured, and n = 9 wells were analyzed per condition from three separate cultures. Means ± SEMs are shown. *p ≤ 0.05. p values are the following: p = 0.0011 (STS), p = 0.0092 (NMDA at 100 μm, 5 min), p = 0.0053 (NMDA at 300 μm, 60 min), p = 0.018 (OGD) versus bax−/−/bok+/+ cultures (ANOVA, post hoc Tukey's test).

Journal: The Journal of Neuroscience

Article Title: Bok Is Not Pro-Apoptotic But Suppresses Poly ADP-Ribose Polymerase-Dependent Cell Death Pathways and Protects against Excitotoxic and Seizure-Induced Neuronal Injury

doi: 10.1523/JNEUROSCI.3780-15.2016

Figure Lengend Snippet: bok/bax double deficiency causes greater sensitivity to NMDA- and OGD-induced neuronal death. A, bax−/−/bok+/+ and bax−/−/bok−/− cortical neurons were used to confirm Bok and Bax protein absence in culture by Western blotting. β-Actin was used as a loading control. B, Western blot and densitometry analysis comparing the levels of several anti-apoptotic members of the Bcl-2 family proteins, including Bcl-2, Bcl-w, Bcl-xL, and Mcl-1 in bax−/−/bok+/+ and bax−/−/bok−/− cortical neurons. β-Actin was used as a loading control. Experiments were repeated three times with different preparations and with similar results. Means ± SEMs are shown. *p ≤ 0.05. p = 0.021 (Mcl-1) versus bax−/−/bok+/+ cultures (ANOVA, post hoc Tukey's test). C, D, Cortical neurons from bax−/−/bok+/+ and bax−/−/bok−/− mice were either treated with STS (100 nm) or exposed to the following: 100 μm NMDA for 5 min; 300 μm NMDA for 60 min; OGD for 90 min; or sham conditions for 90 min. Controls were the pool of DMSO and sham conditions for 24 h and 5 min, respectively. After treatments, cortical neurons were allowed to recover for 24 h. Cell death was assessed by Hoechst 33258 and PI staining, and PI-positive nuclei were scored as dead neurons and expressed as a percentage of the total population. Three subfields containing 300–400 neurons each were captured, and n = 9 wells were analyzed per condition from three separate cultures. Means ± SEMs are shown. *p ≤ 0.05. p values are the following: p = 0.0011 (STS), p = 0.0092 (NMDA at 100 μm, 5 min), p = 0.0053 (NMDA at 300 μm, 60 min), p = 0.018 (OGD) versus bax−/−/bok+/+ cultures (ANOVA, post hoc Tukey's test).

Article Snippet: For the Mcl-1 overexpression single-cell experiments, neurons were cotransfected with a vector expressing Mcl-1 (MC200829; OriGene; Anilkumar et al., 2013 ) and a plasmid expressing enhanced CFP (ECFP-C1; BD Biosciences Clontech) or, for control neurons, transfected with the CFP plasmid only.

Techniques: Western Blot, Staining

Mcl-1 overexpression rescues the mitochondrial phenotype in bok-deficient cortical neurons. bok−/− cortical neurons, transfected with an Mcl-1-overexpressing plasmid or a control empty vector (ECFP) for 48 h and coloaded with TMRM (20 nm) and Fluo-4 AM (3 μm), were exposed to NMDA (100 μm/5 min NMDA) and monitored by confocal microscopy (LSM 710). A, B, Representative Fluo4-AM traces measuring alterations in intracellular Ca2+ influx at the point of stimulation (100 μm/5 min NMDA) and after the initial excitotoxic stimulus. C, D, Representative TMRM traces measuring alterations in Δψm of NMDA-treated bok−/− cortical neurons transfected with Mcl-1-overexpressing plasmid (C) or empty plasmid (D). E, Analysis of the relationship of Fluo-4 fluorescence at the indicated time points compared with peak Fluo-4 AM fluorescence at NMDA exposure. bok−/− cortical neurons transfected with Mcl-1 overexpressing plasmid: n = 56 neurons from n = 6 separate cultures. Empty vector: n = 45 neurons from n = 5 separate cultures. Data are shown as means ± SEMs. *p ≤ 0.05. p values are the following: p = 0.012 (peak), p = 0.029 (Baseline/peak) versus overexpressing Mcl-1 bok−/− cultures (ANOVA, post hoc Tukey's test). F, Average of TMRM fluorescence in bok−/− cortical neurons transfected with Mcl-1-overexpressing plasmid (n = 56; from n = 6 separate cultures) or control vector (n = 45; from n = 5 separate cultures) during and after NMDA excitation. Means ± SEMs are shown. *p ≤ 0.05. p values are the following: p = 0.0035 (45 min), p = 0.0026 (60 min), p = 0.038 (120 min) versus overexpressing Mcl-1 bok−/− cultures (ANOVA, post hoc Tukey's test).

Journal: The Journal of Neuroscience

Article Title: Bok Is Not Pro-Apoptotic But Suppresses Poly ADP-Ribose Polymerase-Dependent Cell Death Pathways and Protects against Excitotoxic and Seizure-Induced Neuronal Injury

doi: 10.1523/JNEUROSCI.3780-15.2016

Figure Lengend Snippet: Mcl-1 overexpression rescues the mitochondrial phenotype in bok-deficient cortical neurons. bok−/− cortical neurons, transfected with an Mcl-1-overexpressing plasmid or a control empty vector (ECFP) for 48 h and coloaded with TMRM (20 nm) and Fluo-4 AM (3 μm), were exposed to NMDA (100 μm/5 min NMDA) and monitored by confocal microscopy (LSM 710). A, B, Representative Fluo4-AM traces measuring alterations in intracellular Ca2+ influx at the point of stimulation (100 μm/5 min NMDA) and after the initial excitotoxic stimulus. C, D, Representative TMRM traces measuring alterations in Δψm of NMDA-treated bok−/− cortical neurons transfected with Mcl-1-overexpressing plasmid (C) or empty plasmid (D). E, Analysis of the relationship of Fluo-4 fluorescence at the indicated time points compared with peak Fluo-4 AM fluorescence at NMDA exposure. bok−/− cortical neurons transfected with Mcl-1 overexpressing plasmid: n = 56 neurons from n = 6 separate cultures. Empty vector: n = 45 neurons from n = 5 separate cultures. Data are shown as means ± SEMs. *p ≤ 0.05. p values are the following: p = 0.012 (peak), p = 0.029 (Baseline/peak) versus overexpressing Mcl-1 bok−/− cultures (ANOVA, post hoc Tukey's test). F, Average of TMRM fluorescence in bok−/− cortical neurons transfected with Mcl-1-overexpressing plasmid (n = 56; from n = 6 separate cultures) or control vector (n = 45; from n = 5 separate cultures) during and after NMDA excitation. Means ± SEMs are shown. *p ≤ 0.05. p values are the following: p = 0.0035 (45 min), p = 0.0026 (60 min), p = 0.038 (120 min) versus overexpressing Mcl-1 bok−/− cultures (ANOVA, post hoc Tukey's test).

Article Snippet: For the Mcl-1 overexpression single-cell experiments, neurons were cotransfected with a vector expressing Mcl-1 (MC200829; OriGene; Anilkumar et al., 2013 ) and a plasmid expressing enhanced CFP (ECFP-C1; BD Biosciences Clontech) or, for control neurons, transfected with the CFP plasmid only.

Techniques: Over Expression, Transfection, Plasmid Preparation, Confocal Microscopy, Fluorescence